REAGENT PREPARATION
Allow all the reagents to equilibrate to room temperature (20 – 25°C) prior to use. Return to
proper storage conditions immediately after use.
Wash buffer
Prepare wash buffer by mixing 60 ml of 20x wash buffer with 1140 ml of distilled or deionized water, which is sufficient for 1 x 96 tests. In case less volume is required, prepare the
desired volume of wash buffer by diluting 1 part of the 20x wash buffer with 19 parts of
distilled or de-ionized water.
Dilution buffer
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