Additionally, water molecules have an important effect on protein adsorbent interactions on biomaterials. The presence of high ionic
strength in the medium affected the hydration properties of proteins
by changing ionic interactions. HA microbeads have got both positive
and negative charges ( Ca2+ and PO43 −),sothe ionic interactionsareimportant. Because this will affect intramolecular, protein-protein and adsorbent-protein interactions. The adsorption capacity decreased with
increasing ionic strength