Fig. 1 Production of recombinant MNEI in G-M17 medium.Coomassie-stained SDS-PAGE (a) and western blot (b) of thetotal protein extract (10 μg) from L. lactis NZ9000 carrying thepNZ8148-MNEI-ll vector (A–D) or the empty pNZ8148 vector (E–F). A:no induction; B: 2 h post-induction; C: 4 h post induction; D: 16 h postinduction; E: no induction; F: 4 h post induction; G: MNEI standard,200 ng; H: MNEI standard, 500 ng