As a β2 sympathomimetic doping, clenbuterol can be detected from urine if it goes
into human body, however, the traditional sample preparation is complicated and
time-consuming. In this paper, LabTech Automated Solid Phase Extraction (SPE)
instrument and LabTech Concentration System were applied to extract and
concentrate clenbuterol from urine sample. The whole automatic sample preparation
process includes solid phase extraction, quantitative concentration and solvent
exchange, which is simple, of high efficiency and of good result reproducibility. The
prepared clenbuterol sample was accurately and fast analyzed by High Performance
Liquid Chromatography (HPLC). The results showed that the calibration curve had a
linear coefficient (R2
) of 0.9998, recovery rate of 89.53%, and RSD of 1.04%.