Hyphothiocyanite has been well studied as food preservatives that was exhibited from the
system namely lactoperoxidase system (LPOS) [1-3]. This antimicrobial agent is able for preserving
food without undesirable side effects [4-6]. Therefore the application is now initialized for industrial
use. The LPOS consists of LPO, H
2
O
2,
and SCN
−
which is able exhibit hypothiocyanite [7]. The
hypothiocyanite has been documented as preservatives in various dairy products such as milk,
cheese, and yogurt, and non-dairy products such as mango and vegetable juice [2, 6, 8-14].
Although it was successfully done for preservatives, previous researchs were unable to inhibit
the proliferation of pathogenic bacteria in milk (fresh and skim milk). This may be explained by the
less power of hyphothiocyanite and the presence of sugar which has been proved as LPO inhibitor
[15]. Then the research was continued to the enhancement of the action of LPOS product. Extract
carrot has been showed to exhibit the enhancement of antimicrobial activity of LPOS [16]
It was documented that the addition of 20-fold diluted carrot extract boosted LPOS
antimicrobial activity from 1.4 to 3.8 log units agaist S. enteritidies. The dilution ratio lower than 10-