Fig. 1. Positive singleplex results for all seven body fluid markers. Shown are examples of electropherograms of singleplex results at neat concentrations of cDNA. Values on the y-axis represent RFUs and the x-axis PCR amplicon size. The height (ht – in RFUs) and size (sz – in base pairs) for the primary marker peaks are noted in the box below each electropherogram. The results for the semen (seminal fluid-specific marker) TGM4 are shown in (A). A secondary peak can also be seen and was typical for neat concentrations. This peak was decreased upon dilution of the cDNA. Results for the blood-specific marker ALAS2 are shown in (B), the saliva-specific marker STATH in (C), the vaginal secretion-specific marker MUC4 in (D), the semen (sperm) specific-marker PRM2 in (E), the saliva-specific marker HTN3 in (F), and the menstrual blood-specific marker MMP7 in (G).