In the above studies, saponin was extracted from Moringa oleifera dried pods and then separated and characterized by thin layer chromatography, HPLC, FTIR and 1H NMR. Elango and Jadhav [26]performed analysis on Moringa oleifera saponins and reported the presence of 13 saponin components at 254nm with Rf values in the range of 0.01-0.87. Our results are in accordance with these results. Jung et al [27]had performed isolation of saponins from Pleurospermum kamtschaticum and showed their inhibitory effect on nitric oxide, prostaglandin E2 and tumor necrosis factor in TLC mobile phase of chloroform-methanol-water (7:3:1, lower phase). The same solvent system is used in the present study to isolate saponin from Moringa oleifera pods.