Corn wine was prepared and used as the
substrate for vinegar production. The cell recycle
system (Figure 2a) involving the flocculate yeast,
S. cerevisiae M30, was used for wine production.
During fermentation, S. cerevisiae M30, which had
flocculated to the bottom, was pumped to the top
of the fermentation vessel at 50 mL/min, which
was the cell recycle rate recommended by Krusong
et al. (2002). The more viable the yeast cells were,
the greater the alcohol content increase in the wine.
As shown in Figure 4, corn wine containing 8.4%
alcohol content and 0.5% acidity was obtained
after 48 h of fermentation at 32°C. The cell recycle