The identification and toxinotyping of
C. perfringens biotypes has traditionally been carried out using an
in vivo toxin neutralization test in mice since 1931 [2]. However,
this conventional method has some disadvantages [6,11]. PCR have
been developed for C. perfringens typing and are a reliable alternative
method to testing in laboratory animals. The various PCR
protocols have been established to genotype C. perfringens isolates
with respect to the genes cpa, cpb, etx, iap, cpe and cpb2
[5e7,11e13].