Mature F0 seeds from transformed lines and from control
plants were air dried, dehusked and, to isolate the endosperm,
polished with emery paper for 8 h on a shaker. In most cases
the transformed endosperms exhibited a clearly notable yellow
color, indicating carotenoid formation.
The pB19hpc single transformants showed a clear 3:1 (colored:noncolored)
segregation pattern, whereas the pZPsC/
pZCycH double transformants showed a wider deviation in
segregation, as expected. To our surprise, the pB19hpc single
transformants, although equipped for lycopene (red) synthesis,were not distinguished in color compared with the pZPsC/
pZCycH double transformants equipped for -carotene (yellow)
synthesis.