Fig. 2. Effect of the content of organic solvent in the running buffer on the
separation. Separation conditions: the running buffer: (a) 1% (w/v) copolymer,
20 mM borax buffer pH 9.2; (b) a + 10% (v/v) methanol; (c) a + 20% (v/v) methanol;
(d) a + 30% (v/v) methanol; (e) a + 40% (v/v) methanol; (f) e + 2% (v/v) 1-butanol.
The other conditions were the same as in Fig. 1.