The chromatographic analysis was performed on a Shimadzu
LC-10 HPLC system, equipped with a reverse-phase column
(Nucleosil C18, ∅4.6mm× 250mm),thermostated at 40 ◦C, and
a UV–Vis detector (Shimadzu SPD 10A, = 280nm). The analytes
were eluted isocratically at a flow rate of 1mL/min, using
a water:acetic acid:n-butanol (350:1:10, v/v/v) mixture as the
mobile phase. The injection volume was 5L per sample.
The chromatographic analysis was performed on a ShimadzuLC-10 HPLC system, equipped with a reverse-phase column(Nucleosil C18, ∅4.6mm× 250mm),thermostated at 40 ◦C, anda UV–Vis detector (Shimadzu SPD 10A, = 280nm). The analyteswere eluted isocratically at a flow rate of 1mL/min, usinga water:acetic acid:n-butanol (350:1:10, v/v/v) mixture as themobile phase. The injection volume was 5L per sample.
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