Considering that the Provisional Tolerable Weekly Intake (PTWI) for Hg was set at 4 mg kg bw (JECFA, 2010), a single consumption of 100 g of mushroom caps cultivated on substrates contaminated with 0.1 mM of Hg by a 70 kg adult would result in exceeding the PTWI by as much as 419% for H. erinaceus (total Hg dose 1.17 mg), 335% for A. bisporus (total Hg dose 0.94 mg), and 186% for P. ostreatus (total Hg dose 0.52 mg).