followed by 35 cycles of 1 min denaturation at 94°C, 1 min annealing at (Ta) according to the primer and 1 min extension at 72°C and finally followed by 10 min of additional extension at 72°C
followed by 35 cycles of 1 min denaturation at 94°C, 1 minannealing at (Ta) according to the primer and 1 minextension at 72°C and finally followed by 10 min ofadditional extension at 72°C