Preparation of mushroom extract
P. ostreatus was dried at 55 C (PO), crushed and made into a fine
powder. Different concentrations of PO (0%, 0.5%, 1.0%, 1.5% and 2%)
were stirred in Milliporewater for 30 min at 70 C and stored at 4 C
overnight. The aqueous extract (POE) was filtered (250 mm) and
used in yogurt production. For antioxidant activity and total
phenolic content, the aqueous extract was freeze-dried (FPOE).