Plant growth and metalloid and metal analysis
The plants were harvested after 12 months for plant growth,
biomass and heavy metal analysis. Whole plant was separated into
root, shoot 1st half, shoot 2nd half and leaf for bioaccumulation
and translocation of As, Cr and Zn analysis. All parts were washed
very carefully with deionized water for four times and blotted
dried. Plant tissues were oven dried at 70 C, and constant dry
weights were recorded. The dried plant materials were ground to
less than 1 mm with a stainless steel mill for metalloid and heavy
metal analysis. The samples were digested in acidic mixture of
HNO3: HClO4, and analysis was done in triplicate by ICP-OES.
Simultaneously, the pot culture soils were analyzed to determine
the concentration of diethylenetriaminepentaacetic acid (DTPA)
extractable As, Cr and Zn using ICP-OES (Lindsay and Norvel,
1978). The DTPA extractable fraction used here represented a bioavailability
of As, Cr and Zn, which is based on the ability of DTPA
to form stable complexes with metallic elements.
Plant growth and metalloid and metal analysis
The plants were harvested after 12 months for plant growth,
biomass and heavy metal analysis. Whole plant was separated into
root, shoot 1st half, shoot 2nd half and leaf for bioaccumulation
and translocation of As, Cr and Zn analysis. All parts were washed
very carefully with deionized water for four times and blotted
dried. Plant tissues were oven dried at 70 C, and constant dry
weights were recorded. The dried plant materials were ground to
less than 1 mm with a stainless steel mill for metalloid and heavy
metal analysis. The samples were digested in acidic mixture of
HNO3: HClO4, and analysis was done in triplicate by ICP-OES.
Simultaneously, the pot culture soils were analyzed to determine
the concentration of diethylenetriaminepentaacetic acid (DTPA)
extractable As, Cr and Zn using ICP-OES (Lindsay and Norvel,
1978). The DTPA extractable fraction used here represented a bioavailability
of As, Cr and Zn, which is based on the ability of DTPA
to form stable complexes with metallic elements.
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