Isolation and enumeration of actinomycetes were performed on different media.
The homogenate was serially diluted and all dilutions were placed on the media.
The isolation was performed on the selective media such as marine sponge agar (MSA)
(raffinose: 10 g; L-histidine: 1 g; ferrous sulphate: 0.01 g; dipotassium hydrogen phosphate: 1 g;
calcium carbonate: 0.5 g; agar: 15 g; sodium chloride: 20 g; aqueous host sponge extract: 100 ml;
double distilled
water: 900 ml; pH 7.8; autoclaved at 15 lbs for 15 min) and
standard media such as Emerson agar (EA) and
modifiedmarine agar (MMA).