for use in
enzyme assays. Livers were minced with a razor blade
and homogenized using a Potter-Elvehjem homogenizer.
A 25% (wt/vol) homogenate was prepared, 5 mL
was removed for further processing to determine AAOX
activity, and the remainder of the 25% homogenate was
used to determine LKR activity, lysine oxidation, and
AASS protein abundance.