2.2. In vitro screening of plant extracts for its antimicrobial activity
against test pathogens
Aqueous extracts of 72 different medicinal plants were obtained as described by Kurucheve et al. (1997). The plant species
used in this study were listed in Table 1. Briefly, 100 g fresh leaves of selected plant species were collected and washed in
distilled water and then in sterile water. Leaves were ground with 100 ml of sterile water (1:1 w/v), using pestle and mortar and filtered through double-layered cheesecloth, followed by centrifugation at 5000 rpm for 10 min at 4 C. Leaf extracts were filter sterilized using a 0.22 lm Millipore filter. Aqueous extracts from tubers, roots, and nuts were similarly prepared and filter sterilized.