Test for adverse effect of A. paniculata supplemented fish feeds To test
whether the supplemented fish feeds had any adverse effect on Nile tilapia, separate
groups of 10 S. agalactiae uninfected fish were fed fish feeds no. 1–12 for a period of
2 weeks. Mortality, appearance, feeding response, and behavior of the fish were
observed daily. Each feeding test was performed in five replicates.