Egg sampleswerecollectedatthefarmlayerimmediatelyafter
egglayingandthenimmediatelytransportedtothelaboratory.The
eggswerebroken,andthealbumenwasseparatedfromtheyolk
with ahouseholdyolkseparator.Foramineextraction,3gofyolk
samples and3gofalbumensampleswereweighedin50-mLpoly-
propylenecentrifugetubes,and7mLoftrichloroaceticacidat
50 gL1 (50 gL1 TCA)wasadded.Thetubeswereagitatedfor
10mininaThermolynetype50800orbitalshaker(Barnstead
Thermolyne,Iowa,USA)at225rpmandthencentrifuged(12,100g)
at 4 °C for21mininachilledBeckmanJ2MCcentrifuge(Beckman
CoulterInc.,California,USA).Aftercentrifugation,thesupernatant
was filteredthroughqualitative filterpaper.Theprocedurewas
repeatedtwomoretimeswiththeadditionof7mLand6mLof
50 gL1 TCAinatotalof20mLofaddedacid.Theresultingextracts
werestoredin0.5mLmicrotubesandfrozenat 20 °C forlater
derivatizationandchromatographicanalysis.
Egg sampleswerecollectedatthefarmlayerimmediatelyafteregglayingandthenimmediatelytransportedtothelaboratory.Theeggswerebroken,andthealbumenwasseparatedfromtheyolkwith ahouseholdyolkseparator.Foramineextraction,3gofyolksamples and3gofalbumensampleswereweighedin50-mLpoly-propylenecentrifugetubes,and7mLoftrichloroaceticacidat50 gL1 (50 gL1 TCA)wasadded.Thetubeswereagitatedfor10mininaThermolynetype50800orbitalshaker(BarnsteadThermolyne,Iowa,USA)at225rpmandthencentrifuged(12,100g)at 4 °C for21mininachilledBeckmanJ2MCcentrifuge(BeckmanCoulterInc.,California,USA).Aftercentrifugation,thesupernatantwas filteredthroughqualitative filterpaper.Theprocedurewasrepeatedtwomoretimeswiththeadditionof7mLand6mLof50 gL1 TCAinatotalof20mLofaddedacid.Theresultingextractswerestoredin0.5mLmicrotubesandfrozenat 20 °C forlaterderivatizationandchromatographicanalysis.
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sampleswerecollectedatthefarmlayerimmediatelyafter ไข่egglayingandthenimmediatelytransportedtothelaboratory .eggswerebroken andthealbumenwasseparatedfromtheyolk ,กับ ahouseholdyolkseparator foramineextraction 3gofyolk , .and3gofalbumensampleswereweighedin50 mlpoly - ตัวอย่างpropylenecentrifugetubes and7mloftrichloroaceticacidat ,50 gl1 ( 50 gl1 TCA ) wasadded.thetubeswereagitatedfor10mininathermolynetype50800orbitalshaker ( บาร์นสเต็ดthermolyne , Iowa , USA ) at225rpmandthencentrifuged ( 12100g )ที่ 4 ° C ( for21mininachilledbeckmanj2mccentrifuge เบคแมนcoulterinc , California , USA ) aftercentrifugation thesupernatant ,คือ filteredthroughqualitative theprocedurewas filterpaper .repeatedtwomoretimeswiththeadditionof7mland6mlof50 gl1 theresultingextracts tcainatotalof20mlofaddedacid .werestoredin0.5mlmicrotubesandfrozenat 20 ° C forlaterderivatizationandchromatographicanalysis .
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