3. Explain how Coombs reagents are produced.
4. Explain the role of Coombs control cells in antiglobulin testing.
5. Explain the mode of action of the Coombs control cells.
8. Explain why a lavender top tube is best for DAT testing.
After the tube has been filled with blood, immediately invert the tube several times in order to prevent coagulation.
Applications
Coombs reagent (also known as Coombs antiglobulin or antihuman globulin) is used in both the direct Coombs test and the indirect Coombs test. Coombs reagent is antihuman globulin. It is made by injecting human globulin into animals, which produce polyclonal antibodies specific for human immunoglobulins and human complement system factors. More specific Coombs reagents or monoclonal antibodies can be used.