The antioxidant and radical scavenging activity of DTSPI wasassessed by using two in vitro free radical models, namely,DPPH and ABTS+. According to Li et al. (2015), radicalscavenging assays have been widely used to evaluate the antioxidantproperty of various antioxidants. When DPPH andABTS radicals encounter a proton-donating substance such asan antioxidant, the radical is scavenged and the absorbance isreduced by changing the color (Chalamaiah et al. 2015). Inthis study, we can note that the DPPH free radical scavengingactivity of protein isolate was increased dose-dependently.Intarasirisawat et al. (2012) reported that the DPPH scavengingactivity increased with increasing concentrations. Theterm IC50 was used to evaluate the radical scavenging effects.Lower IC50 indicates higher free radical scavenging effect. AnIC50 value for protein isolate was estimated to be about40.89 μg/mL (Fig. 3a). Protein isolate exhibited also an effectiveradical cation scavenging activity and was an effectiveABTS•+ radical scavenger in a concentration-dependent manner(25–200 μg/mL). IC50 in this assay was 18.45 μg/mL(Fig. 3b). The results reveal the considerable antioxidant capacityof the protein isolate which can be considered as aninexpensive potential source of high added value with powerfulnatural antioxidants. Tomato and derivative products arerecognized as a valuable source of so-called functional foodbecause of their natural antioxidant content such as lycopeneand β-carotene. According to Zhang et al. (2015), the tomatocontains also a higher content of glutamate, the procurer of theglutamic acid, compared to other vegetables. In fact, aminoacids were considered important for the antioxidant activity ofprotein hydrolysates. For instance, amino acids with aromaticresidues could donate protons to electron-deficient radicals (Liet al. 2015). According to Pieri et al. (2008), the aromaticresidues such as tyrosine, phenylalanine, and tryptophan engagedin van der Waals interactions are known to have theability to donate and receive protons due to their unique chemicalstructure.
The antioxidant and radical scavenging activity of DTSPI was<br>assessed by using two in vitro free radical models, namely,<br>DPPH and ABTS+. According to Li et al. (2015), radical<br>scavenging assays have been widely used to evaluate the antioxidant<br>property of various antioxidants. When DPPH and<br>ABTS radicals encounter a proton-donating substance such as<br>an antioxidant, the radical is scavenged and the absorbance is<br>reduced by changing the color (Chalamaiah et al. 2015). In<br>this study, we can note that the DPPH free radical scavenging<br>activity of protein isolate was increased dose-dependently.<br>Intarasirisawat et al. (2012) reported that the DPPH scavenging<br>activity increased with increasing concentrations. The<br>term IC50 was used to evaluate the radical scavenging effects.<br>Lower IC50 indicates higher free radical scavenging effect. An<br>IC50 value for protein isolate was estimated to be about<br>40.89 μg/mL (Fig. 3a). Protein isolate exhibited also an effective<br>radical cation scavenging activity and was an effective<br>ABTS•+ radical scavenger in a concentration-dependent manner<br>(25–200 μg/mL). IC50 in this assay was 18.45 μg/mL<br>(Fig. 3b). The results reveal the considerable antioxidant capacity<br>of the protein isolate which can be considered as an<br>inexpensive potential source of high added value with powerful<br>natural antioxidants. Tomato and derivative products are<br>recognized as a valuable source of so-called functional food<br>because of their natural antioxidant content such as lycopene<br>and β-carotene. According to Zhang et al. (2015), the tomato<br>contains also a higher content of glutamate, the procurer of the<br>glutamic acid, compared to other vegetables. In fact, amino<br>acids were considered important for the antioxidant activity of<br>protein hydrolysates. For instance, amino acids with aromatic<br>residues could donate protons to electron-deficient radicals (Li<br>et al. 2015). According to Pieri et al. (2008), the aromatic<br>residues such as tyrosine, phenylalanine, and tryptophan engaged<br>in van der Waals interactions are known to have the<br>ความสามารถในการบริจาคและรับโปรตอนเนื่องจากสารเคมีที่ไม่ซ้ำกัน<br>โครงสร้าง
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