in mitochondria, citrate synthase converts acetyl-CoA and oxaloacetate
to citric acid. Further on in the tricarboxylic acid cycle (TCA),
citrate is converted into cis-aconitate which is being transported
back into cytosol and then decarboxylated to itaconate. A graphical
presentation of the presumed pathway is given in Fig. 2 (Jaklitsch
et al., 1991). The efficient production of itaconic acid will depend
on the sufficient fluxes of metabolites between two cell compartments,
as was also addressed by Pedersen et al. for amylase production
in Aspergillus oryzae