Increasing fuel crisis and environmental concerns, advert biomass as a potential alternative. Nevertheless pretreatment of biomass is great challenge, still considerable efforts enduring for commercializing the pretreatment technology. During pretreatment, ratio of solid (biomass)/ to catalyst (acid/alkali) plays an important role; a connection between solid to liquid ratio and concentration of released sugars was observed along with increased enzymatic digestibility of the pretreated corncobs. Solid to liquid ratio (1:20) significantly detached the xylan part and improved the enzymatic hydrolysis for reducing sugar production. Compared with H2SO4 pretreatment, NaOH pretreatment of corncob substantially produced low reducing sugars. Moreover, enzymatic hydrolysate from H2SO4-pretreated corncobs contained higher concentrations of fermentable sugars which is more suitable for the subsequent fermentation process for value added chemicals production. Future efforts on optimization of S/L ratio improves the sugar production from corncobs.