UHT system was sterilized by means of superheated water in all
process circuit at 136 °C for 45 min prior to treatment. After treatment,
the equipment was cleaned following the CIP procedure (cleaning-inplace).
First of all, it was passed through UHT circuit alkaline solution
(3% NaOH) at 95 °C for 30 min and acid solution (2% HNO3) at
45–50 °C for 20 min. Finally, a disinfecting solution (0.3%) prepared
with acetic and peracetic acids and hydrogen peroxide was used.