The in vitro cytotoxicity of the newly synthesized target compounds 4–36 against MCF-7, SW-1990, SMMC-7721, and HUVEC cell lines was measured by an assay was based on the cleavage of the yellow tetrazolium salt MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) to form purple formazan crystals in viable cells.