Various studies had been conducted over the past decade to understand the impact of medium to long term climate alteration to soil microbiota. However, responses of the microbial community to short-term temperature and water variation were rarely tested in laboratory-based microcosms, especially for tropical soil. Using our controlled microcosm setup, we showed that the bacterial
community in a tropical soil reacted rapidly to variations in temperature and water (the greatest community difference was observed in the Week 2 microcosms), and that the new community
equilibrium was achieved in just 4 weeks (as by then all treatments exhibited similar community structure). We showed that the number of phyla decreased with increasing temperature and water
content, with Firmicutes dominating at higher temperatures and water content. This correlated with a higher abundance of nifH gene detected in microcosms incubated at 35 C and reduction of
nosZ gene with increase of temperature. Observations on specific gene numbers could be used as a proxy to reflect the ecological function that the gene mediate. We are aware that changes to soil bacterial community and function in the field may differ due to the effects of other environmental factors (e.g. solar radiation, influence from plants and animals). Nevertheless, this microcosm study elucidates the short-term effects of two major environmental drivers, temperature and water content, on a tropical soil bacterial community and some functional genes, under controlled laboratory settings.
Various studies had been conducted over the past decade to understand the impact of medium to long term climate alteration to soil microbiota. However, responses of the microbial community to short-term temperature and water variation were rarely tested in laboratory-based microcosms, especially for tropical soil. Using our controlled microcosm setup, we showed that the bacterialcommunity in a tropical soil reacted rapidly to variations in temperature and water (the greatest community difference was observed in the Week 2 microcosms), and that the new communityequilibrium was achieved in just 4 weeks (as by then all treatments exhibited similar community structure). We showed that the number of phyla decreased with increasing temperature and watercontent, with Firmicutes dominating at higher temperatures and water content. This correlated with a higher abundance of nifH gene detected in microcosms incubated at 35 C and reduction ofnosZ gene with increase of temperature. Observations on specific gene numbers could be used as a proxy to reflect the ecological function that the gene mediate. We are aware that changes to soil bacterial community and function in the field may differ due to the effects of other environmental factors (e.g. solar radiation, influence from plants and animals). Nevertheless, this microcosm study elucidates the short-term effects of two major environmental drivers, temperature and water content, on a tropical soil bacterial community and some functional genes, under controlled laboratory settings.
การแปล กรุณารอสักครู่..