Four cascade enzymatic reactions catalysed with polymerase,
ATP sulfurylase, luciferase and apyrase are used in pyrosequencing
chemistry. Pyrosequencing chemistry described in our previous
research allows the use of an inexpensive light sensor photodiode
array in a portable pyrosequencer because of increased sensitivity.
The concentration of apyrase is critical in the reaction system especially
when the concentration of polymerase, luciferase and ATP
sulfurylase are tailored to ensure high sensitivity and low background
signal.