Callus segments cut into the size of 15–20 mm3 in capacity
were transplanted on MS medium (pH 6) containing 0.8%
agar and 3% sucrose, supplemented with the different kinds
and concentrations of plant growth regulators 0.2 mg/l and
0.5 mg/l NAA in combination with three concentrations of
BAP (1, 2 and 3 mg/l) or each BAP alone (1 and 2 mg/l) and
cultivated at (25± 1) C under a 14 h photoperiod by coolwhite
fluorescent lights (36.0 lmol m2 s1) for 28 days.