The process used for production of grape juice presented high yield without the pressing the fruit. The temperature of 60 °C with addition of pectinase resulted in an increase on the yield and decrease of turbidity. The use of enzyme decreased the concentration of catechins and increased the content of procyanidin B1 and B2. The temperature of maceration of 60 °C contributed to the higher extraction of phenolic compounds, mainly anthocyanins. The combination of temperature 60 °C with the use of enzymatic poll resulted in higher extraction of all analyzed phenolic compounds, increase of almost all organic acids and decrease of acetic acid content. The antioxidant activity was high and can be related to the contents of anthocyanins, total phenolics, catechins, caffeic acid, cinnamic acid and gallic acid. Among the quantified bioactive compounds, the caffeic acid, procyanidin B1 and trans-resveratrol presented higher contents compared to juices of other regions. The analysis of principal components was efficient to show the effect of maceration treatments, giving evidence of positive effect of temperature and pectinases addition on the extraction of desirable grape compounds. A high quality juice was obtained in industrial scale, contributing to the process development taking into account the bioactive properties of the product.