The filtered supernatants were used to measure fructose,
glucose, sucrose and malic acid using a Dionex P680 HPLC system
(Dionex Corporation, CA, USA). Sugars were detected by a Shodex
RI-101 refractive index detector with reference cell maintained at
40C. A Transgenomic CARB Sep Coregel 87C column (300 mm
7.8 mm i.d., 10lm particle size) with a guard column cartridge
(Transgenomic CARB Sep Coregel 87C cartridge) was used. The column was maintained at 85C with a Dionex TCC-100 thermostated
column compartment. Degassed, distilled, deionized water at a
flow rate of 0.6 ml/min was used as the mobile phase. The injection
volume was 10lL. Malic acid was detected using a Dionex
PDA-100 detector. The Inertsil ODS-3 column (250 mm4.6 mm
i.d., 5lm particle size) with a guard column cartridge (Sunchrom
C18 cartridge) was used. The column was maintained at 40C.
Samples were eluted with 0.02 mol/L KH2PO4 solution with pH
2.4. The flow rate was 0.8 ml/min. Eluted compounds were
detected by UV absorbance at 210 nm. The Chromeleon chromatography data system was used to integrate peak areas according
to external standard solution calibrations (reagents from Sigma
Chemical Co., Castle Hill, NSW, Australia). Sugar and acid concentrations were expressed in mg/g fresh weight (FW)
The filtered supernatants were used to measure fructose,glucose, sucrose and malic acid using a Dionex P680 HPLC system(Dionex Corporation, CA, USA). Sugars were detected by a ShodexRI-101 refractive index detector with reference cell maintained at40C. A Transgenomic CARB Sep Coregel 87C column (300 mm7.8 mm i.d., 10lm particle size) with a guard column cartridge(Transgenomic CARB Sep Coregel 87C cartridge) was used. The column was maintained at 85C with a Dionex TCC-100 thermostatedcolumn compartment. Degassed, distilled, deionized water at aflow rate of 0.6 ml/min was used as the mobile phase. The injectionvolume was 10lL. Malic acid was detected using a DionexPDA-100 detector. The Inertsil ODS-3 column (250 mm4.6 mmi.d., 5lm particle size) with a guard column cartridge (SunchromC18 cartridge) was used. The column was maintained at 40C.Samples were eluted with 0.02 mol/L KH2PO4 solution with pH2.4. The flow rate was 0.8 ml/min. Eluted compounds weredetected by UV absorbance at 210 nm. The Chromeleon chromatography data system was used to integrate peak areas accordingto external standard solution calibrations (reagents from SigmaChemical Co., Castle Hill, NSW, Australia). Sugar and acid concentrations were expressed in mg/g fresh weight (FW)
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กรอง supernatants ถูกใช้เพื่อวัดฟรักโทส
กลูโคส ซูโครสและกรด malic โดยใช้ระบบ HPLC p680 DIONEX
( DIONEX Corporation , CA , USA ) น้ำตาลที่ถูกตรวจพบโดย shodex
ri-101 ดรรชนีหักเหด้วยเครื่องตรวจจับเซลล์อ้างอิงไว้ที่ 40
C transgenomic carb ก.ย. coregel 87c คอลัมน์ ( 300 มม.
7.8 มิลลิเมตร บัตรประชาชน ขนาดอนุภาค 10lm ) กับยามตลับ
คอลัมน์( transgenomic carb ก.ย. coregel 87c ตลับ ) ใช้ คอลัมน์ไว้ที่ 85 C กับ DIONEX tcc-100 thermostated
คอลัมน์ช่อง . degassed กลั่นคล้ายเนื้อเยื่อประสาน , อัตราการไหลของน้ำที่
0.6 มิลลิลิตรต่อนาทีใช้เป็นเฟสเคลื่อนที่ ปริมาณการฉีด
คือ 10ll . กรดมาลิกที่ตรวจพบการใช้ DIONEX
pda-100 เครื่องตรวจจับ คอลัมน์ inertsil ods-3 ( 250 มม. 4.6 mm
บัตรประจำตัว 5lm particle size) with a guard column cartridge (Sunchrom
C18 cartridge) was used. The column was maintained at 40C.
Samples were eluted with 0.02 mol/L KH2PO4 solution with pH
2.4. The flow rate was 0.8 ml/min. Eluted compounds were
detected by UV absorbance at 210 nm. The Chromeleon chromatography data system was used to integrate peak areas according
to external standard solution calibrations (reagents from Sigma
Chemical Co., Castle Hill, NSW, Australia). Sugar and acid concentrations were expressed in mg/g fresh weight (FW)
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