3.2 Callus Formation
The combinations of NAA and BAP effected
on different growth callus as shown in Table 2. The
highest callus fresh weight was observed on MS
medium supplemented with 2 mg/L NAA and 0.5
mg/L BAP. Two color types of callus were obtained;
red friable callus (Figure 2A) and green-yellow
friable callus (Figure 2B) which referred to as Type
I and Type II, respectively. Type I callus was found
mostly in the experiment whereas Type II was only
on MS medium supplemented with 5 mg/L NAA
with 1 mg/L, 5 mg/L and 10 mg/L BAP.
3.3 Cell Viability (TTC assay) of Callus
After using tetrazolium assay, the efficiency of
callus viability was showed in Table 3. The result
showed that more than 50% of viability callus was
observed as in Figure 3.
4. Discussion
The outer surfaces of roots generally are
infested with spores and other microbial cells. To
reduce the amount of contamination on explants
derived from this part, root explants should be
washed under running tap water for 45 minutes
before treatment with a sterilized solution. This
technique was successful for removing
contamination from plant tissue and using in
micropropagation techniques [7]. After washing, the