This study showed that the flocculating S. cerevisiae strain KF-7 is appropriate for establishing continuous ethanol fermentation process using sugar beet raw juice and thick juice as feed, with the advantages of improved productivity and thermotolerant fermentation. Single-stage fermentation was sufficient for sugar beet raw juice. Two-stage fermentationwas proper for sugar beet thick juice. Although ethanol concentration fluctuated in the second reactor (R2) due to stress of high ethanol concentration (80 g L1), it was overcome by feeding fresh yeast cells.