3.5. Knockdown of OsLTPL36 resulted in delayed embryo
development and decreased seed germination rate
The knockdown of OsLTPL36 resulted in reduced seed setting
rate andgrains withhigher chalkiness rate. The seeddevelopmental
course was investigated by cytological section of seeds.
Histological analysis showed thatthe embryo development process
was delayed in OsLTPL36-RNAi lines (Fig. 5B1–B5, refer to type
I). In WT, the embryonic shoot apical meristem differentiated after
late globular stage (3 DAP), the first leaf primordium emerged at
5 DAP, and the second and third leaf primordial at 7 DAP, subsequently
(Fig. 5A1–A5). In OsLTPL36-RNAi lines, the embryos stayed
at late globular embryo stage in 5 DAP (Fig. 5B2), and at 7 DAP
the embryo just emerged the first leaf primordium in OsLTPL36-
RNAi lines (Fig. 5B3). In addition, abnormal morphology of embryo
were observed in OsLTPL36-RNAi lines (Fig. 5C1–C5, refer to type
II). This result indicated that OsLTPL36 might be involved in embryo
development