The efficacy of added probiotic bacteria depends on
dose level. Their viability must be maintained throughout
the product’s shelf-life and they must survive the gut environment.
To exert positive health effects, they have to
establish themselves in certain numbers in the gastrointestinal
tract (Kailasapathy & Sultana, 2003). A standard,
requiring a minimum of 106–107 colony forming units per
gram (cfu g1) of L. acidophilus and/or Bifidobacteria in
fermented milk products, has been introduced by several
food organizations world-wide