Experimental animal and regime
L. rohita fingerlings with an average weight of 9.50 0.50 gwere
procured from Malad Fish Farm, Maharashtra, India. The fishes
were acclimatized for 15 days in laboratory condition in 1000 L FRP
tanks at 25e27 C, under continuous aeration. The fishes were fed
with control pelleted diet at the rate of 3% of body weight twice a
day.