The 2% concentrated essential oil was extracted and prepared by the Medicinal Plants Research Center of North Khorasan.The extraction process was done using a Clevenger hydro distillation device on the aerial parts of the pelargonium graveolens. The extracted oil was kept in a refrigerator in appropriate airtight opaque containers. The Ethics Committee granted permission for the study and issued a letter of referral to North Khorasan University of Medical Sciences in 2012.
The sampling phase then began at Bint-al-Hoda Hospital.