1. To day I would like to take this opportunity to present the topic Bioflocculant produced from bacteria for decolorization,Cr removal and swine wastewater application.
2. From introduction Flocculation is the process by which the suspension charge neutralization and integration into larger particles, called the flock. By the addition of a chemical group called Flocculant. When the sediment is larger weight was more. It can sink down to the bottom of the tank more quickly.
3. Bioflocculant is sediments-biological,which can be produced from microorganisms such as bacteria,fungi,algae species. By microorganism each type is produced biological-coagulant elements are different
4. the flocculant chemical have to risk is a cancer but Bioflocculant have sencurity and can also use of remove dye, Remove heary metals and Remove of suspended solids
5. Objective the first one To isolate the bacteria to produce compounds bioflocculant the second one To study the conditions for the precipitation of bioflocculant isolated the third one To study the color removal in synthetic wastewater, removal of chromium in the wastewater plant and turbidity removal in sewage farm pigs
6. For Method of separating infections Bioflocculant - producing medium as the following Method of separating infections divided into two parts .that is Culture medium and Seed medium.
7. Isolation of bacteria use serial dilution method from this figure .
8. Microorganisms isolated all 17 species, isolated in the flask 50 ml. Put into culture medium Incubated for 2 days at 30 degree celsius and shaked at 150 Revolutions per minute and Put into seed medium Incubated for 3 days at 35 degree celsius and shaked at 150 Revolutions per minute
9. Measurement of Flocculating Efficiency ( kaolin clay susensions method) seed medium
CaCl_2 and Kaolin Adjusted the pH 7.0-7.5 substance for 1 min.
Then rest for 10 min lead sediment at lower than the liquid 1 cm, measure the light absorption. By elisa reader The absorbance of the solution at a wavelength of 550 nm.
10. The flocculating efficiency was calculated as follows Flocculating efficiency (%) as absorbance of blank control minus absorbance of supernatant divided by absorbance of blank control times 100.
11. Extraction Flocculant, measuring the growth of cells xn11and xn7 Centrifuged at 5000 Revolutions per minute substances for a period of 30 min It 2 parts supernatant to Measurement the absorbance at 660 nanometer sediment Soak them in cold ethanol at 4 degree celsius (to accelerate precipitation) After 12 hours the material centrifuged at 5000 Revolutions per minute /min for a period of 30 min. Dried at 40 degree celsius Will be Bioflocculant.
12. Effects of various salts, pH and thermal stability on flocculating efficiency
one. Study the effects of the infection xn11and xn7 included At different concentrations
two. Study the effects of positive ions included โพแทสเซียมไอออน โซเดียมไอออน แมกนีเซียมไอออน อะลูมิเนียมไอออน ไอร์ออนไอออน แคลเซียมไอออน
three. Study the effect of pH ranged 3 - 11
four. Study the effect of the temperature , at 20 – 40 degree celsius
13. The study of wastewater treatment using bioflocculant Dyes removal Mix the ingredients as follows (ชี้ๆ) Colour analysis with a colourimeter and Mix for 1 minute, then Set aside 10 minutes to precipitate 2 section Supernatant Colour analysis and Sediment Flocculating analysis
14. Removal of chromium in the wastewater in electroplating Mix the ingredients as follows Wastewater 50 ml Bioflocculant 1 ml Adjusted pH Chromium analysis with a AAS (Atomic Absorption spectroscopy) and Mix for 1 minute, then Set aside 10 minutes to precipitate 2 section Supernatant Chromium analysis and Sediment Flocculating analysis
15. Turbidity removal in swine wastewater Mix the ingredients as follows (ชี้ๆ) Turbidity analysis with a Turbidity Meter and Centrifuged at 150 Revolutions per minute for a period of 2 min, then slowly rotated at 60 Revolutions per minute for 1 min. 2 section Supernatant Turbidity analysis and sediment Flocculating analysis
16. Surface characterization of strains xn11 and xn7 As shown in the table.
17. Effect of initial pH of medium, inoculum volume and culture temperature on flocculating efficiency The table shows that initial pH 5 , inoculum volume 0.3 plus 0.3 and culture temperature 30 degree celsius on the most flocculating efficiency .
18. Figure 1 illustrates the growth, flocculating efficiency and pH variation curves with time. During the initial growth phase, the production of bioflocculant by xn11 + xn7 was almost parallel to the cell growth and a large amount of the bioflocculant was achieved at the stationary phase (3-5 d) with the flocculating efficiency over 96%.
19. Effect of pH and various cations on the flocculating efficiency of xn11 and xn7 The cation could stimulate the flocculation by neutralization and destabilization of residual negative charges of functional g