Samples from various points in the opiate alkaloid extraction process were analysed using this approach and the results
were compared to those of a standard GlaxoSmithKline method based on ion-pairing HPLC and UV-absorbance detection.
The samples were analysed on-site immediately after collection to minimise degradation.
Dilution of the samples was required to operate within the linear calibration range, due to the sensitivity of tris(2,2-bipyridyl)ruthenium(III) chemiluminescence reagent.