Dryad's Saddle (Polyporus squamosus) was cultured on a substrate comprised of 75% waste cotton burrs from the USDA located in Lubbock Tex. and 24% cottonseed hulls from Planters Cotton Mill in Arkansas. The substrate was buffered with 1%gypsum and was mixed at a 100% moisture content. The cottonseed hulls and cotton burrs were sterilized by soaking in a 35% hydrogen peroxide solution for 25 minutes. The gypsum, cotton burrs, and cotton seed hulls, were than poured into 5 cubic footflexible opaque bags with breathable membranes and inoculated using 2 liters of liquid inoculum. Bags were incubated for 45 days at 21 degrees Celsius.
Referring to FIG. 7, small punctures, with a diameter of approximately 0.125''were created every 12'' along a 12''.times.12'' grid creating a series of orifices 3 along a set of grid lines 9.
Referring to FIG. 8, An enclosure 4, sized 2''.times.12''.times.24'', was placed over the series of orifices and firmly affixed to the flexible incubation bag 1. Though not pictured, each of the puncture points was covered in a similarenclosure to enclosure 4. Blue light at an intensity of 40 lumens at a distance of one meter from flexible bag 1 was used to initiate creation of the primordium, with additional primordium forming at each puncture site.
These primordium were grown out under additional incident light at a intensity of about 1,000 lumens illuminating an area of about 100 square feet, cycled on/off at 12 hour intervals to encourage primordium 5 to undertake stipe elongation alongthe width, height and depth of enclosure 4. This light exposure, in addition to exposure to CO2 concentrations of between 1500-3000 PPM encouraged primordia to grow along the 12''.times.24''.times.1'' rectangular section beginning to fill enclosure 4with fungal tissue 9.