2.4.3. Protease production
It was done as per the protocols of Bhattacharya et al. (2009).
Actinomycetes were streaked on casein agar and incubated at
28 2 C for four days. At the end of the incubation, the plates were
observed for halo zone around the colonies, which indicates the
presence of protease. Observations were recorded on a 04 rating
scale as follows: 0 ¼ no change; 1 ¼ positive; 2 ¼ halo zone of
1e3 mm; 3 ¼ halo zone of 4e6 mm and 4 ¼ halo zone of 7 mm and
above.