The developmentofsustainable,bio-ba

The developmentofsustainable,bio-basedtechnologiestoconvertsolarenergyandcarbondioxideinto
fuels isagrandchallenge.Acorepartofthischallengeistoproduceafuelthatiscompatiblewiththe
existingtransportationinfrastructure.Thistaskisfurthercompoundedbythecommercialdesireto
separatethefuelfromthebiotechnologicalhost.Basedonitsfuelcharacteristics,1-octanolwas
identified asanattractivemetabolictargetwithdiesel-likeproperties.Wethereforeengineereda
synthetic pathwayspecifically forthebiosynthesisof1-octanolin Escherichiacoli BL21(DE3)byover-
expressionofthreeenzymes(thioesterase,carboxylicacidreductaseandaldehydereductase)andone
maturation factor(phosphopantetheinyltransferase).Inductionofthispathwayinashake flask resulted
in 4.4mg1-octanolL
1 h
1 which exceededtheproductivityofpreviouslyengineeredstrains.
Furthermore,themajority(73%)ofthefattyalcoholwaslocalisedwithinthemediawithoutthe
addition ofdetergentorsolventoverlay.Thedeletionof acrA reduced theproductionandexcretionof
1-octanol by3-foldrelativetothewild-type,suggestingthattheAcrAB–TolCcomplexmaybe
responsible forthemajorityofproductefflux. Thisstudypresents1-octanolasapotentialfueltarget
that canbesynthesisedandnaturallyaccumulatedwithinthemediausingengineeredmicrobes.
& 2014TheAuthors.PublishedbyElsevierB.V.InternationalMetabolicEngineeringSociety.Thisisan
open accessarticleundertheCCBYlicense

ผลลัพธ์ (ไทย) 1:

Developmentofsustainable, basedtechnologiestoconvertsolarenergyandcarbondioxideinto ทางชีวภาพเชื้อ isagrandchallengeAcorepartofthischallengeistoproduceafuelthatiscompatiblewiththeexistingtransportationinfrastructureThistaskisfurthercompoundedbythecommercialdesiretoseparatethefuelfromthebiotechnologicalhostBasedonitsfuelcharacteristics, 1 octanolwasระบุ asanattractivemetabolictargetwithdiesel-likepropertiesWethereforeengineeredaสังเคราะห์ pathwayspecifically forthebiosynthesisof1 octanolin Escherichiacoli BL21 (DE3) byover -expressionofthreeenzymes (thioesterase, carboxylicacidreductaseandaldehydereductase) และหนึ่งพ่อแม่ factor(phosphopantetheinyltransferase)หนาว Inductionofthispathwayinashake ผลใน 4.4mg1-octanolL 1 h 1 exceededtheproductivityofpreviouslyengineeredstrains ที่นอกจากนี้ themajority(73%) ofthefattyalcoholwaslocalisedwithinthemediawithouttheนอกจากนี้ ofdetergentorsolventoverlayThedeletionof acrA ลด theproductionandexcretionof1-octanol by3-foldrelativetothewild-ชนิด suggestingthattheAcrAB – TolCcomplexmaybeforthemajorityofproductefflux รับผิดชอบ Thisstudypresents1-octanolasapotentialfueltargetที่ canbesynthesisedandnaturallyaccumulatedwithinthemediausingengineeredmicrobesและ 2014TheAuthors.PublishedbyElsevierB.V.InternationalMetabolicEngineeringSociety.Thisisanเปิด accessarticleundertheCCBYlicense

ผลลัพธ์ (ไทย) 2:

developmentofsustainable ชีวภาพ basedtechnologiestoconvertsolarenergyandcarbondioxideinto
เชื้อเพลิง asanattractivemetabolictargetwithdiesel-likeproperties.Wethereforeengineereda สังเคราะห์ pathwayspecifically forthebiosynthesisof1-octanolin Escherichiacoli ปัจจัย (phosphopantetheinyltransferase) ขวด .Inductionofthispathwayinashake ผลใน 4.4mg1-octanolL? 1 ชั่วโมง 1 ซึ่ง ofdetergentorsolventoverlay.Thedeletionof Acra ลด theproductionandexcretionof 1-octanol BY3-foldrelativetothewild ประเภท suggestingthattheAcrAB-TolCcomplexmaybe รับผิดชอบ forthemajorityofproductefflux Thisstudypresents1-octanolasapotentialfueltarget ที่ canbesynthesisedandnaturallyaccumulatedwithinthemediausingengineeredmicrobes. & 2014TheAuthors.PublishedbyElsevierB.V.InternationalMetabolicEngineeringSociety.Thisisan เปิด accessarticleundertheCCBYlicense

ผลลัพธ์ (ไทย) 3:

การ developmentofsustainable ชีวภาพ basedtechnologiestoconvertsolarenergyandcarbondioxideinto
เชื้อเพลิง isagrandchallenge . acorepartofthischallengeistoproduceafuelthatiscompatiblewiththe
existingtransportationinfrastructure . thistaskisfurthercompoundedbythecommercialdesireto basedonitsfuelcharacteristics 1-octanolwas
,
separatethefuelfromthebiotechnologicalhost .ระบุ asanattractivemetabolictargetwithdiesel likeproperties . wethereforeengineereda
สังเคราะห์ pathwayspecifically forthebiosynthesisof1 octanolin escherichiacoli BL21 ( DE3 ) byover -
expressionofthreeenzymes ( thioesterase carboxylicacidreductaseandaldehydereductase
, ) และเป็นปัจจัย ( phosphopantetheinyltransferase ) inductionofthispathwayinashake ขวด )
4 .4mg1 octanoll
1 H
1 ซึ่ง exceededtheproductivityofpreviouslyengineeredstrains .
นอกจากนี้ส่วนใหญ่ ( 73% ) ofthefattyalcoholwaslocalisedwithinthemediawithoutthe
2 ofdetergentorsolventoverlay . thedeletionof ACRA ลดลง theproductionandexcretionof
เส้น by3 foldrelativetothewild ชนิด suggestingthattheacrab – tolccomplexmaybe
รับผิดชอบ forthemajorityofproductefflux .thisstudypresents1 octanolasapotentialfueltarget ที่ canbesynthesisedandnaturallyaccumulatedwithinthemediausingengineeredmicrobes
.
& 2014theauthors . publishedbyelsevierb . v.internationalmetabolicengineeringsociety thisisan
เปิด accessarticleundertheccbylicense .