drial genes have become available . The quest has been for ‘universal primers’ applicable to a broad range of taxa provided a comprehensive compilation of primer sequences for the amplification of virtually every gene encoded in insect
mtDNA. In this paper we provide DNA sequence for four new primers for amplification of insect mtDNA gene frag-
ments to supplement those presented by Simon . We also present the results of PCR amplification of portions of six mitochondria1 genes from ten orders of insects and a tick using the four new primers in combination with previously published primers. The primers used in this study amplify portions of the two rRNA genes, cytochrome
c oxidase I gene, NADH4 and NADH5 dehydrogenase genes and the entire tRNA gene.