Diluted inoculum (0.4 ml, 105 CFU/ml) was uniformly spread onto individual NA plates using sterile cotton swabs. Sterile oxford cups (6 mm in diameter) were then placed on the agar medium and filled with 200 ll of sugarcane bagasse extract (20 mg/ml).
Diluted inoculum (0.4 ml, 105 CFU/ml) was uniformly spread ontoindividual NA plates using sterile cotton swabs. Sterile oxford cups(6 mm in diameter) were then placed on the agar medium andfilled with 200 ll of sugarcane bagasse extract (20 mg/ml).