ทานอล 70%
The SEM imagery was performed at CSIRO Black Mountain Laboratories in Canberra, ACT. The ovules were placed in 70% dry ethanol and dehydrated in 10% steps for 30 min per step to 100% ethanol. The 100% ethanol was changed twice and the tissue was left for up to 8 h in ethanol. The dehydrated ovules were dried in an Autosamdri-815 automatic critical point drier (Tousimis Research Corporation, USA) according to the protocol of Talbot and White (2013). Individual ovules were dissected and mounted flat on aluminium stubs with double-sided sticky carbon tabs (ProSciTech, AUS) and photographed in a Ziess EVO LS 15 Scanning Electron Microscope. The ovules were imaged with a back scatter detector under a vacuum of 30 Pa at 20 °C with Extra High Voltage of 20.0 kV and a spot size of 500 μm.