One gram of each soil sample was shaken in 100 mL of phosphate buffered saline.
Then, serial dilutions of each suspension were plated on King’s B agar (KB) medium: 20 g L-1 bactopeptone, 1 g L-1 K2HPO4, 1.5 g L-1 MgSO4 7 H2O, 10 mL L-1 glycerol, 15 g L-1 agar. After incubation at 28 C for 24 h, representative colonies were selected for further studies.