Water hyacinth is one of the most invasive aquatic weeds of Kerala. A survey was conducted in the waterways of four southern districts of Kerala to document the fungal pathogens of water hyacinth. Out of the 21 fungi recorded, 17 were pathogenic. Among these, Myrothecium advena Sacc. is a new report on water hyacinth. Furthermore, M. advena and Fusarium pallidoroseum (Cooke) Sacc. caused more than 50% infection of the weed and hold promise as biocontrol agents of water hyacinth