Tulasnella isolate to seedlings for 49 days, it was found that mycelium formed pelotons in the cortical cells of roots in the form of intact and degenerate pelotons. Significant differences were detected between the control group and the experimental group treated with Tulasnella isolates in dry weight and fresh weight of plants respectively. The study firstly demonstrated that two different strains of Tulasnella vary in their ability to facilitate germination/development of D. officinale in vitro.