Fig. 5. Electrophoretic mobility shift assay of MaC2H2-1/2 binding to the biotin-labeled A[C/G]T sequence presented in MaACS1 and MaACO1 promoters. The purified GST or
recombinant GST-MaC2H2-1/2 protein was mixed with probes, and the protein-DNA complexes were separated on native polyacrylamide gels. ++ or +++ indicate increasing
amounts unlabeled probes for competition.